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real-time PCTiggo

来源:http://www.17930ip.com 作者:威尼斯城所有登入网址 时间:2020-02-07 20:04

核心提示:hi,I am going to do real-time PCR using Smart Cycler, but I have never done this experiment, I do not know how to desighi,I am going to do real-time PCR using Smart Cycler, but I have never done this experiment, I do not know how to design primers,such as the length of primers,the size of PCR product and the annealing temperature et al.If you happen to know the knowledge about it, would you like to sharing with me?Thanks a lot!

-geness-

Here you can find SmartCycler specific information on primer design for real-time PCR. Additional guidelines can be found here If you are going to use SYBR Green I dye, primer design is similar to regular RT-PCR. However, since SYBR Green I dye will detect all double-stranded DNA, including non-specific reaction products, optimization of the reaction conditions for each primer set are very important.SAGE

-sage-

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