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4斤二个的,原代细胞抽离的骨干方式

来源:http://www.17930ip.com 作者:威尼斯城所有登入网址 时间:2020-04-15 09:41

核心提示:人或动物体内的组织,将其剪碎至1mm3的组织块,再采用如下方法进行原代细胞分离培养:一、悬浮细胞的分离方法1、将血液seline; line-height: 1.5;">seline; line-height: 2;">人或动物体内的组织,将其剪碎至seline; line-height: 2;">1mmseline;">3的组织块,再采用如下方法进行原代细胞分离培养:

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; text-align: left; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 100元一斤的太空草莓、180元一个的“梨子”、100元一斤的猕猴桃您见过吗?据了解,如今一批湖北产的高端水果崛起,它们身价倍增,价格超出普通水果10多倍。它们有的有异域血统,有的有着特殊营养成分,这些尖板眼水果如何在荆楚大地生根结果,高价背后隐藏着怎样的秘密?楚天金报记者近日进行了探访,给“果粉”带来惊喜。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; text-indent: 0pt; line-height: 2;">

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 探访

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-indent: 0pt; text-align: center; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; text-indent: 0pt; line-height: 2;">图片 1

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 4斤一个的“梨子”卖180元

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-indent: 0pt; text-align: center; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; text-indent: 0pt; line-height: 2;">

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 白色的草莓您见过吗?记者昨日就看到这一稀罕物。在黄陂罗汉街木兰莓产业文化园,记者看到大棚里试种的半亩白草莓已陆续挂果,个头和一般红草莓差不多,但更加圆润,预计半个月后可面市。“今年武汉市民吃草莓有口福了,今年试种的10多个草莓新品种将大量上市,无论口感和营养价值都提升许多。”黄陂草莓种植协会会长江晋笑着对记者说。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">一、悬浮细胞的分离方法

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 木兰莓产业文化园技术顾问刘伟介绍,白草莓是日本改良品种,曾经在浙江、山东等地都试种失败,但唯独在黄陂种植成功,果实汁液多,耐储存,有水蜜桃的味道,由于品种稀缺,上市后市价至少在100元一斤。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">1、将血液、羊水、胸水或腹水等悬液直接转至离心管中,1000rpm/分钟离心5分钟。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 记者看到,10多个大棚均为双层薄膜,有来自日本、美国等地的多个品种。江晋说,今年黄陂试种的新品种中,有太空草莓,一个重达2两,比一般草莓重一倍,且耐储存,亩产可达1万斤。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">2、去掉上清,离心沉淀用无钙、镁的PBS清洗后1000rpm/分钟离心5分钟。此步重复两次。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 江晋说,草莓是否好吃和个头长相关系不大,主要是品种的原因。以前武汉草莓品种多是大路货,口感很清淡,糖度不够。这几年开始普及“法拉第”、“红艳”等新品种,含糖量增加一倍,所谓“牛奶草莓”就是“红艳”。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">3、用培养基重悬,调整适当细胞浓度后分瓶培养。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 随后,记者驱车一个多小时来到孝感孝南区三汊镇石板沟水库附近,发现这里300多亩“梨树”上挂满大个金黄色水果,“梨树”其实比一般梨树矮很多,每棵树上挂10个左右的黄金果,单个有的重达4斤。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">4、如选用悬液中某种细胞,可采用离心后的细胞分层液收获目的细胞。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 湖北天圆生态发展公司总裁杨志军笑着对记者说,这是在全国独一无二的土耳其金果,吃起来有梨子、苹果、哈密瓜和香蕉4种水果的综合口感。记者品尝一口,脆甜爽口、满嘴盈香。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">二、实体组织材料的分离方法

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 杨志军介绍,这种金果原产在土耳其,8年前由我国林果专家引进到国内,去年独家在孝感试种成功,其特点是肉多核小,含糖量高达18%,比一般梨子高一倍,一般单个果重在2斤以上,最大的有4.8斤。价格也不低,2斤多的50元一个,3斤以上的180元一个。更重要的是,金果含有一种多肽酶,有抗氧化防癌保健功效,且常温下可以放置6至8个月。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">机械分散法

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 湖北天价果王一年增近10种

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">1、纤维成分很少的脑组织,部分胚胎组织,用剪刀剪碎至1mm3的组织块。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 除了草莓和金果外,记者近日探访得知,新奇特的天价水果今年在我省猛增近10个品种,包括猕猴桃、柑橘、贡枣、蓝莓、樱桃等品种。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">2、用PBS清洗两次后

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 上月,记者在宜昌看到,一款贡橘皮薄肉甜汁多,吸引了众人的眼球。种植的土老憨公司负责人介绍,这是目前最贵的橘子,是与中国农业大学、华中农业大学等院校联合研制的新品种,市场零售价5元一个。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">①用吸管吹打,分散组织细胞。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 在武汉植物园,新研制的“金玉”猕猴桃个头小巧玲珑,表皮为金黄色,切开横面,只见果肉呈迷人的金黄色,一口咬下去,肉质鲜嫩、满口香甜,比普通的猕猴桃在口感上更加馥郁多汁。猕猴桃育种专家钟彩虹介绍,“金玉”为高果糖、高有机酸品种,营养价值是普通猕猴桃的1.5倍,预计明年可在汉批量上市,最低售价在100元一斤。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">②或将已充分剪碎分散的组织放在注射器内,使细胞通过针头压出。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 在黄梅大别山蓝莓基地,种植了2000多亩来自美国的兔眼蓝莓,它是北美北纬30度得克萨斯州的优良蓝莓品种,生长期比普通蓝莓长1至2个月,花青素含量是一般蓝莓的3-5倍。种植户周文胜介绍,这种兔眼蓝莓制成100%果汁,每斤售价在100元,喝起来对眼睛很有益。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">③或在不锈钢纱网内用钝物压挤使细胞从网孔中压挤出。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 揭秘

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">3、收集细胞转入离心管中,1000rpm/分钟离心5分钟。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 有机种植产量低致身价涨

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">4、去上清,加入含血清的培养基,重悬后移至培养瓶中培养。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 为何新型鄂产水果这么贵?杨志军介绍,首先品种优、口感佳,营养价值也高,其次引进成本较高,此外种植完全是有机绿色无污染,不打农药,用诱蛾灯灭虫。“和金果同类型的韩国新高梨、日本秋月梨每斤价格都在60元。今后我们计划在恩施种植1万亩富硒金果,目前已在海拔1850米的山区试种成功,其口感更佳,随着种植面积扩大,价格会降低。”杨志军对记者说。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">消化分离法

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 相关农业专家介绍,天价果王多有异域血统,湖北有山有水有平原,独特的气候和土壤适合外来的水果品种生根挂果。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">1、酶消化分离法

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 江晋介绍,黄陂的土壤能接受国外新品种草莓,今年黄陂新增1万亩草莓种植面积,品种更新换代,俏销北上广,新品种草莓售价在50至100元一斤,贵的原因就是绿色环保,不仅果实圆润,吃起来更加香甜。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">①细胞间质较少的软组织,如肝、肾、甲状腺、羊膜、胚胎组织、上皮组织等,用Hanks液清洗组织三次,剪成碎块大小为4毫米左右。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 高昂的研发成本也是贵的原因。钟彩虹说,“金玉”猕猴桃是从该园“红阳”猕猴桃的后代中选育出来的,花了10年心血,而培育首个国家猕猴桃种质资源圃花了38年时间,研发一个新品种起码要淘汰数十个旧的品种,需花费大量人力物力。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">②再用Hanks液洗2-3次以除去血球和脂肪组织。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 思考

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">③加入0.25%的胰蛋白酶,摇匀后放4℃过夜。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 果市迎来健康消费时代

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">④次日再用Hanks液洗涤,弃去上清,共洗2-3次。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 面对记者采访,鄂产猕猴桃、金果等研发负责人均表示,要种植世界上最好吃的水果,鄂产金果目前已出口美国、新加坡、韩国等地。而我省遍地开花的高端水果现象也引起专家关注,思考如何形成品牌做大做强。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">⑤加入少量含血清的培养基吹打分散,细胞计数,按适当的浓度分瓶培养。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 武汉现代农业教育中心专家彭火炬分析,随着时代变化,水果消费潜力巨大。根据省政府《湖北省食物与营养发展实施计划》,提出食物消费量目标,到2020年,全省人均全年肉类消费25-30公斤,水果消费55-65公斤,吃水果为吃肉的两倍。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">2、非酶消化法

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 我省是全国水果生产大省之一,去年果园面积发展到580万亩。今年上半年脐橙、桃、葡萄等已经上市的水果质高价稳,柑橘、梨子等秋季水果的品质也好于往年。“宜昌蜜橘”、“秭归脐橙”、“砂梨”等湖北三大名牌,实现了湖北水果品牌重要突破。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">①把组织块剪碎,呈1mm3大小的组织块。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 然而,水果产业发展也面临一些挑战。相关专家分析,果园生态环境比较脆弱,附加值高的水果还不多,水果精深加工不够,大品牌的龙头企业还不多,今后需要用现代科学技术改造水果产业,用现代经营形式提升水果的品牌和美誉度。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">②将碎组织块在平皿中用无钙镁PBS洗2-3次。

seline; line-height: 1.8; color: rgb; font-family: Arial, Helvetica, sans-serif; text-indent: 0px; background-image: initial; background-attachment: initial; background-size: initial; background-origin: initial; background-clip: initial; background-position: initial; background-repeat: initial;"> 武汉市农委市场处处长胡体良认为,今年出现的高端水果抢占市场,是个很好的变化,说明水果正在经历传统产业转型的嬗变,告别低价位竞争,走向市民需求的有机健康消费时代。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">③加入消化液(胰蛋白酶或胶原酶或EDTA)于37℃水浴中作用适当时间(中间可轻摇1~2次),若组织块膨松呈絮状可终止,若变化不大可更换一次消化液,继续消化直至膨松絮状为止。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">④弃去上清,加入含有钙、镁离子的培养基中止消化反应,并洗涤2-3次后,加入完全培养基。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">⑤用吸管吹打,使细胞充分散开后用纱网过滤后分瓶培养。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">原代细胞培养

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">原代培养是直接从生物体获取细胞进行培养。由于细胞刚刚从活体组织分离出来,故更接近于生物体内的生活状态。这一方法可为研究生物体细胞的生长、代谢、繁殖提供有力的手段,同时也为以后传代培养创造条件。利用此方法还可直接服务于临床实践。例如,用从手术中切除的肿瘤细胞进行原代培养,然后用该培养细胞进行抗癌药物的筛选,根据肿瘤细胞对加入的化疗药物的敏感性来帮助选择最有效的化疗方案,有可能起到增强疗效、降低副作用的作用。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">原代细胞的传代、保存

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">(seline; line-height: 2;">1)传代:依椐细胞的生长状态,生长的细胞1:2或1:3进行传代。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">(seline; line-height: 2;">2)保存:DMSO+培养液+血清

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">按下列顺序降温:室温seline; line-height: 2;">→4℃→冰箱冷冻室→超低温冰箱→液氮。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">原代细胞的复苏

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">(seline; line-height: 2;">1)取出细胞,迅速放入37℃温水中快速解冻。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">(seline; line-height: 2;">2)吸出细胞悬液,并加10倍以上培养液。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">(seline; line-height: 2;">3)用培养液适当稀释后接种培养瓶,放入37℃ CO2培养箱静置培养。

seline; color: rgb; font-family: "Microsoft YaHei", SimSun, Arial; text-align: justify; text-indent: 0pt; background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">seline; line-height: 2;">美国seline; line-height: 2;">ScienCell公司提供seline; color: rgb; text-decoration: none;">seline; color: rgb;">原代细胞及原代细胞培养全套培养试剂,包括seline; color: rgb; text-decoration: none;">seline; color: rgb;">血清,seline; color: rgb; text-decoration: none;">seline; color: rgb;">培养基,seline; color: rgb; text-decoration: none;">seline; color: rgb;">胰酶消化液,seline; color: rgb; text-decoration: none;">seline; color: rgb;">胰酶中和液,seline; color: rgb; text-decoration: none;">seline; color: rgb;">细胞冻存液等。北京裕恒丰公司是美国ScienCell公司国内首家全国代理,竭诚为您提供优质的产品和专业的服务。

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